Biologist III

Responsibilities:

• Impeccable lab practices, including aseptic cell line/primary cell maintenance and stimulation. Self-motivated with excellent attention to detail.
• Demonstrated ability to optimize cell culture conditions, phenotypical assays, Western Blot, migration/contraction assays (fibroblasts), viability assays, qPCR, ELISA/multiplex protein assays, and flow cytometry.
• Experience with human cell types relevant to tissue homeostasis such as fibroblast, epithelial cells, and macrophages.
• Familiar with complex in vitro models such as co-culture, tissue explants, Transwells, organoids, 3D/microfluidic/chip systems a plus.
• Ability to analyze, plot, interpret, and present data in team meetings.
• -Aptitude to communicate in a virtual environment and a hybrid office/virtual setting.
• Proficient at maintaining accurate and well-organized records and laboratory electronic notebooks.

Requirements:

• 5 + years hands-on academia/industry experience.
• BS/MS degree in Biology, Biochemistry, Immunology, Bioengineering/Biomedical engineering, or Medical/Pharmaceutical sciences.
• Primary cell culture and stimulation.
• Molecular biology techniques (e.g. ELISA, qPCR, RNAseq, Western Blot, flow cytometry).
• Pharmacological treatment with small/large molecules and siRNAs.
• Data analysis including statistics.